Composite

Part:BBa_K2259073:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-02)


Rop protein with anderson (0.15) promoter for global plasmid copy number control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Rop protein sequence was mutated in order to avoid prohibited restriction sites.


Source

Sequence of rop was taken from wildtype ColE1 plasmid on Genbank.

References